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KMID : 0357119870090010017
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Korean Journal of Immunology
1987 Volume.9 No. 1 p.17 ~ p.26
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Suppressive Effect of Intravaginal Administration of Human Seminal Plasma on Immune Responses to Human Sperms and Sheep Red Blood Cells
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Abstract
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A number of evidence has been accumulated to show that human seminal plasma (HSP) can suppress the immune responeses in vitro and in vivo. Several immunosuppressive factors have been identified from HSP and it has been proposed that these factors play a critical role in preventing sensitization of females to spermantigens after insemination. The purpose of the present study is to answer the q question whether HSI´ is able to suppress in vivo immune responses induced by some antigens such as human sperms and sheep red blood cells(SRBC) which are introduced into the vagina of females. In this study the author used an experimental model in which mice were immunized intravaginally with human sperms and SRBC, and delayed-type hypersensitivity reacion (DTH) to these antigens was eva-
luated by footpad swelling reaction. Repeated simultaneous intravaginal administrations of HSP with human sperms or SRBC significantly suppressed the DTH but failed to suppress the antibody formation to these antigens. However, intravaginal administration of a high molecular weigt fraction(G? 200 F, greater than 200 KD) which was partially purified by ammonium sulfate precipitation, DEAE- cellulose and Sephadex G -200 chromatography from HSP resulted in a significant suppression of DTH as well as antibody formation to SRBC. Furthermore, when various concentrations of G?200 F were ad-ministered intraperitoneally, a dose-dependent suppression of both DTH and antibody formation to SR BC was observed. These results provide the evidence that in vivo immunosuppressive activity of HSP is mediated by a high molecular weight fraction of greater than 200 KD and strongly support the concept that HSP can prevent sensitization of females to sperm antigens after insemination.
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